Compositions and methods for production of immunoglobulins

C - Chemistry – Metallurgy – 12 – N

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C12N 15/13 (2006.01) C07H 21/00 (2006.01) C07K 7/08 (2006.01) C07K 14/47 (2006.01) C07K 16/00 (2006.01) C07K 16/12 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01) C12P 19/34 (2006.01) C12P 21/08 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2616859

Provided are oligonucleotides for isolating human antibody cDNAs from cells or cell lines, such as hybridomas. The invention also provides cDNAs that encode at least one provided CDR of heavy chain or a light chain of a human monoclonal antibody that binds to B. anthracis protective antigen; and cDNAs that encode at least one provided CDR of a heavy chain or a light chain of a human monoclonal antibody that binds to B. anthracis lethal factor. The invention further provides expression vectors that contain one or more cDNAs isolated according to the methods of the invention, host cells expressing one or more inventive cDNAs, and transgenic plants and animals that express one or more inventive cDNAs. In certain embodiments of the invention the expression system is a plant-based expression system. The invention further provides antibody compositions comprising one or more antibodies produced by expressing a cDNA isolated according to the methods of the invention in a suitable expression system. Additionally encompassed in the invention are kits containing one or more of provided compositions, as well as methods of production and use of provided compositions.

La présente invention concerne des oligonucléotides permettant d'isoler des ADNc d'anticorps humains à partir de cellules ou de lignées cellulaires, telles les hybridomes. L'invention porte aussi sur des ADNc qui codent au moins un CDR de chaîne lourde ou légère d'un anticorps monoclonal humain qui se lie à l'antigène protégeant contre le Bacillus anthracis ; et des ADNc qui codent au moins un CDR d'une chaîne lourde ou légère d'un anticorps monoclonal humain qui se lie au facteur létal du B. anthracis. L'invention a également trait à des vecteurs d'expression qui renferment un ou plusieurs ADNc isolés selon les procédés de l'invention, à des cellules hôtes exprimant un ou plusieurs ADNc de l'invention, ainsi que des plantes et des animaux transgéniques qui expriment un ou plusieurs ADNc de l'invention. Dans certains modes de réalisation de l'invention, le système d'expression correspond à un système d'expression reposant sur les plantes. En outre, l'invention se rapporte à des compositions d'anticorps comprenant un ou plusieurs anticorps produits en exprimant un ADNc isolé selon les procédés de l'invention dans un système d'expression approprié. De plus, l'invention porte sur des kits contenant une ou plusieurs compositions, ainsi que des procédés de production et l'utilisation desdites compositions.

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