C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/63 (2006.01) C07K 14/245 (2006.01) C12N 15/31 (2006.01) C12N 15/62 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2324974
The present invention relates to compositions and methods for secretion of functional proteins in a soluble form by host cells. In particular, the invention relates to membrane targeting and translocation proteins, MttA, MttB and MttC and to variants and homologs thereof. The membrane targeting and translocation proteins are useful in targeting protein expression to the periplasm of gram negative bacteria and to extracellular media of other host cells. Such expression allows secretion of expressed proteins of interest in a functional and soluble form, thus facilitating purification and increasing the yield of functional proteins of interest.
La présente invention concerne des compositions et des méthodes permettant à des cellules hôtes de sécréter des protéines fonctionnelles sous forme soluble. Cette invention concerne plus particulièrement le ciblage membranaire, des protéines de translocation ci-après dénommées MttA, MttB, et MttC, et les variants et homologues de celles-ci. Ce ciblage membranaire et ces protéines de translocation permettent de cibler l'expression protéique sur le périplasme d'une bactérie gram négative et sur le milieu extracellulaire d'autres cellules hôtes. Cette expression permet notamment la sécrétion des protéines exprimées recherchées, sous une forme fonctionnelle et soluble, ce qui facilite la purification de ces protéines fonctionnelles recherchées et augmente leur production.
Turner Raymond Joseph
Weiner Joel Hirsch
Bennett Jones Llp
The Governors Of The University Of Alberta
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