Compositions and methods for random nucleic acid mutagenesis

Details Compositions and methods for random nucleic acid mutagenesis Compositions and methods for random nucleic acid mutagenesis

C - Chemistry, Metallurgy

12

N

C12N 9/12 (2006.01) C12N 15/10 (2006.01) C12N 15/54 (2006.01) C12N 15/63 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2448628

The invention relates to compositions and methods for nucleic acid PCR mutagenisis using novel error-prone DNA polymerases and a PCR enhancing factor. The invention also relates to compositions and methods for nucleic mutagenesis with two or more DNA polymerases lacking or exhibiting reduced exonuclease activity. The invention further relates to kit format of said compositions for PCR mutagenesis.

L'invention concerne des compositions et des procédés destinés à la mutagenèse par PCR d'acide nucléique au moyen de nouvelles ADN polymérases sujettes à l'erreur et d'un facteur amplificateur PCR. L'invention concerne également des compositions et des procédés pour la mutagenèse nucléique avec au moins deux ADN polymérases présentant une activité d'exonucléase réduite ou inexistante. L'invention concerne par ailleurs des formats de nécessaires de ces compositions pour la mutagenèse par PCR.

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