C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/66 (2006.01) C07K 14/245 (2006.01) C12N 15/09 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01)
Patent
CA 2444195
The present invention relates generally to recombinant DNA technology. The invention relates more specifically to compositions and methods for recombinational cloning of nucleic acid molecules using recombination systems. In particular, the invention relates to compositions comprising one or more Fis proteins and one or more additional components used for recombinational cloning (such as one or more recombination proteins). The invention further relates to the use of the above compositions in methods of recombinational cloning of nucleic acid molecules. The invention also relates to isolated nucleic acid molecules produced by methods of the invention, to vectors comprising such nucleic acid molecules, and to host cells comprising such nucleic acid molecules and vectors.
La présente invention concerne de manière générale la technique de l'ADN recombinant. L'invention concerne plus particulièrement des compositions et des procédés destinés au clonage recombinant des molécules d'acides nucléiques utilisant des systèmes recombinants. Elle concerne tout particulièrement des compositions comprenant une ou plusieurs protéines Fis et un ou plusieurs composants supplémentaires utilisés pour le clonage recombinant (tels qu'une ou plusieurs protéines recombinantes). L'invention concerne aussi l'utilisation de ces compositions dans des procédés de clonage recombinant de molécules d'acides nucléiques. L'invention concerne également des molécules isolées d'acides nucléiques, produites par les procédés de l'invention, des vecteurs comprenant ces molécules d'acides nucléiques et des cellules hôtes comprenant ces vecteurs et molécules d'acides nucléiques.
Byrd Devon R. N.
Esposito Dominic
Invitrogen Corporation
Osler Hoskin & Harcourt Llp
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