Compositions and methods for regulation of plant...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 14/415 (2006.01) C07K 14/47 (2006.01) C07K 19/00 (2006.01) C12N 5/04 (2006.01) C12N 9/10 (2006.01) C12N 15/29 (2006.01) C12N 15/82 (2006.01) C12P 17/06 (2006.01)

Patent

CA 2482597

Disclosed herein are zinc finger proteins that bind to targt sites in a plant gammatocopherol methyl transferase (GMT) gene; compositions comprising these GMT-targeted zinc finger proteins and methods of making and using such zinc finger proteins. Also disclosed are methods for modulating the alpha- tocopherol content in various plant organs, particularly seeds, in transgenic plants.

L'invention concerne des protéines à doigt de zinc qui se lient à des sites ciblés dans un gène végétal de gamma-tocophérol méthyltransférase (GMT), des compositions comprenant lesdites protéines à doigt de zinc ciblées par GMT et des procédés permettant de préparer et d'utiliser lesdites protéines à doigt de zinc. L'invention concerne également des procédés de modulation du contenu alpha-tocophérol dans divers organismes végétaux, notamment dans des graines et dans des plantes transgéniques.

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