Compositions and methods for releasing nucleic acids from...

Details Compositions and methods for releasing nucleic acids from... Compositions and methods for releasing nucleic acids from...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2573905

Methods of isolating nucleic acids are disclosed comprising binding the nucleic acid to solid phase binding materials and releasing the bound nucleic acid from the solid phase by elution with a novel reagent composition. Compositions feature a high ionic strength buffer or an added hydrophilic organic co-solvent or both. Preferred solid phase materials for use with the methods and compositions of the invention comprise a quaternary onium nucleic acid binding portion.

La présente invention a pour objet des méthodes permettant d'isoler des acides nucléiques. Il s'agit, dans ces méthodes, de lier chimiquement l'acide nucléique à des matériaux en phase solide, puis de libérer l'acide nucléique lié à la phase solide par élution à l'aide d'une nouvelle composition de réactifs. Les compositions concernées contiennent soit un tampon de force ionique élevée, soit un co-solvant organique hydrophile, soit ces deux éléments. Les matériaux en phase solide préférés utilisés avec les méthodes et les compositions décrites dans la présente invention comportent un site de liaison de l'acide nucléique de type onium quaternaire.

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