Compositions and methods for reverse transcriptase...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/70 (2006.01) A61K 31/513 (2006.01) A61K 31/575 (2006.01) A61K 31/655 (2006.01) C12P 19/34 (2006.01) C12Q 1/40 (2006.01) C12Q 1/42 (2006.01) C12Q 1/52 (2006.01) C12Q 1/68 (2006.01) G01N 33/68 (2006.01) G01N 33/72 (2006.01)

Patent

CA 2528419

The invention can be summarized as follows. The present invention provides compositions, methods and kits useful for the amplification of nucleic acid molecules from human (3-retrovirus by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a one or two-step real time RT-PCR procedure using reverse transcriptase, DNA polymerase, or a combination of both enzymes. The invention provides for the rapid and efficient amplification, detection and quantification of human 0-retrovirus nucleic acids.

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