Compositions and methods for reverse transcription

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/22 (2006.01) C12N 9/12 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2561325

The present invention provides compositions and methods for high fidelity cDNA synthesis. In particular, the composition of the present invention contains a first enzyme exhibiting a reverse transcriptase activity and a second enzyme comprising a 3'-5' exonuclease activity.

La présente invention concerne des compositions et des méthodes de synthèse ADNc d'une grande fidélité. En particulier, la composition de la présente invention contient une première enzyme présentant une activité transcriptase inverse et une seconde enzyme présentant une activité exonucléase 3'-5'.

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