Compositions and methods for screening drug libraries

Details Compositions and methods for screening drug libraries Compositions and methods for screening drug libraries

G - Physics

01

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G01N 33/53 (2006.01) C07K 1/04 (2006.01) C07K 14/705 (2006.01) C07K 14/715 (2006.01) G01N 33/537 (2006.01) G01N 33/543 (2006.01) G01N 33/554 (2006.01) G01N 33/566 (2006.01) G01N 33/68 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2250870

A method of screening for binding partners of a specific molecule. The method employs a chimeric protein having at least two different binding regions; one containing at least a portion of the specific molecule or an analog thereof, and the other containing a binding region of an immunoglobulin chain. In a preferred embodiment, the method is used for rapidly screening member compounds of a combinatorial library for potential biological activity.

L'invention a trait à une technique de prospection de partenaires de liaison d'une molécule spécifique. Cette technique fait intervenir une protéine chimère possédant au moins deux régions de fixation différentes, l'une contenant au moins une partie de la molécule spécifique ou un analogue de celle-ci et l'autre, une région de fixation d'une chaîne d'immunoglobuline. Dans un mode de réalisation préféré, on utilise cette technique pour trier rapidement des composés appartenant à une banque combinatoire aux fins d'une éventuelle activité biologique.

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