Compositions and methods for screening using populations of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2516326

Methods and compositions for the detection, identification, and quantification of compounds of interest in a sample are provided. The compositions and methods include arrays and kits comprising a population of surrogate antibodies that bind compounds of interest. The surrogate antibodies are modified aptamers and can be immobilized on to a solid support by means of an interaction between a recognition nucleotide sequence comprised in the surrogate antibody and a capture nucleotide sequence comprised in a capture probe attached to the solid support. Also provided are methods of using the arrays for research and clinical diagnostics, drug discovery, environmental testing (esp. PCBp), food testing, and testing for the use of agents of biological and chemical warfare.

L'invention concerne des procédés et des compositions permettant de détecter, d'identifier et de quantifier des composés intéressants dans un échantillon. Ces compositions et procédés comprennent des réseaux et des trousses comportant une population d'anticorps de substitution qui se lient à des composés intéressants. On immobilise les anticorps de substitution sur un support solide au moyen de l'interaction entre une séquence nucléotidique de reconnaissance comprise dans ledit anticorps et une séquence nucléotidique de capture comprise dans une sonde de capture fixée au support solide. L'invention concerne aussi des procédés d'utilisation des réseaux à des fins de diagnostic clinique et de recherche, dans la recherche de médicaments, les essais environmentaux ou alimentaires et les essais en matière d'utilisation d'agents biologiques et chimiques de guerre.

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