Compositions and methods for targeted in vitro and in vivo...

A - Human Necessities – 61 – K

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A61K 47/00 (2006.01)

Patent

CA 2556248

A composition comprising intact minicells that contain a drug molecule is useful for targeted drug delivery. One targeted drug delivery method employs bispecific ligands, comprising a first arm that carries specificity for a bacterially derived minicell surface structure and a second arm that carries specificity for a mammalian cell surface receptor, to target drug-loaded minicells to specific mammalian cells and to cause endocytosis of the minicells by the mammalian cells. Another drug delivery method exploits the natural ability of phagocytic mammalian cells to engulf minicells without the use of bispecific ligands.

Cette invention concerne une composition renfermant des minicellules intactes qui contiennent une molécule de médicament, laquelle composition est utilisée dans la libération de médicaments ciblée. Un procédé de libération de médicaments ciblée fait appel à des ligands bispécifiques, qui comprennent un premier bras qui porte la spécificité pour la structure d'une surface de minicellule dérivée d'une bactérie et un second bras qui porte la spécificité pour un récepteur de surface de cellule mammifère, pour cibler des minicellules chargées de médicament sur des cellules mammifères spécifiques et pour provoquer l'endocytose des minicellules par les cellules mammifères. Un autre procédé de libération de médicaments exploite la capacité naturelle de cellules mammifères phagocytiques à envahir les minicellules sans l'aide de ligands bispécifiques.

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