Compositions and methods for the analysis of degraded...

Details Compositions and methods for the analysis of degraded... Compositions and methods for the analysis of degraded...

C - Chemistry, Metallurgy

07

H

C07H 21/04 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2607454

The invention relates to compositions and methods for gene expression analysis. In some embodiments, the invention provides compositions and methods for amplifying targets in a degraded nucleic acid sample. In some embodiments, the invention provides methods for determining the quality of nucleic acids (e.g., the degree of degradation) in a nucleic acid sample. The invention also provides methods for producing a gene expression profile from a degraded RNA sample.

L'invention concerne des compositions et des procédés d'analyse de l'expression génique. Dans certains modes de réalisation, l'invention porte sur des compositions et des procédés d'amplification de cibles dans un échantillon d'acide nucléique dégradé. Dans d'autres modes de réalisation, elle se rapporte à des procédés de détermination de la qualité des acides nucléiques (par exemple le degré de dégradation) dans un échantillon d'acide nucléique. Cette invention concerne également des procédés de fabrication d'un profil d'expression génique à partir d'un échantillon d'ARN dégradé.

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