Compositions and methods for the detection of mycobacterium...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12N 15/31 (2006.01)

Patent

CA 2255624

The featured invention discloses and claims olignonucleotide hybridization assay probes and helper oligonucleotides which are designed to be complementary to specific regions of M. kansasii rRNA or the DNA encoding it, or to an oligonucleotide or nucleic acid comprising, consisting essentially of, or consisting of, a M. kansasii rRNA or rDNA nucleotide sequence. The hybridization probes of the present invention are designed to hybridize to a target nucleic acid in a region of the molecule having a specific target nucleotide sequence under conditions which allow the selective detection of the target nucleic acid. The probes are further designed to detect M. kansasii typical as well as atypical strains. The present invention also discloses and claims double-stranded nucleic acid hybrid molecules formed between the hybridization probes and their specific target nucleic acids.

Selon la présente invention, on décrit et on revendique des sondes de dosage d'hybridation oligonucléotidiques et des oligonucléotides auxiliaires conçus pour être complémentaires à des régions spécifiques d'ARNr de M. Kansasii ou l'ADN qui le code, ou à un oligonucléotide ou un acide nucléique comprenant une séquence nucléotidique d'ADNr ou d'ARNr de M. kansasii, ou essentiellement ou entièrement composé d'une telle séquence. Les sondes d'hybridation selon la présente invention sont conçues pour s'hybrider à un acide nucléique cible dans une région de la molécule présentant une séquence nucléotidique cible spécifique dans des conditions permettant la détection sélective de l'acide nucléique cible. Ces sondes sont de plus conçues pour détecter des souches de M. Kansasii typiques aussi bien qu'atypiques. On décrit et on revendique également des molécules hybrides d'acide nucléique double-brin formées entre les sondes d'hybridation et leurs acides nucléiques cibles spécifiques.

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