Compositions and methods for the identification of...

A - Human Necessities – 61 – K

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A61K 31/7105 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2699859

Methods of identifying inhibitors of retroviral propagation, tRNA used in the methods, and kits, including the tRNA, which can be used in the methods, are disclosed. Methods of treating or preventing retroviral infections by administering an effective amount of the inhibitors, and pharmaceutical compositions including the inhibitors, are also disclosed. The methods involve forming a mixture comprising a linear sequence of a tRNA anticodon stem loop fragment that is not capable of forming a stem-loop, a target nucleic acid molecule capable of binding to the tRNA anticodon stem loop fragment, and a test compound. The mixture is incubated under conditions that allow binding of the tRNA anticodon stem loop fragment and the target nucleic acid molecule in the absence of the test compound. Assays can then be performed that detect whether or not the test compound inhibits the binding of the tRNA anticodon stem loop fragment and the target nucleic acid molecule.

L'invention concerne des procédés d'identification d'inhibiteurs de propagation rétrovirale, des ARNt utilisés dans les procédés, et des kits, comprenant les ARNt, qui peuvent être utilisés dans les procédés. Elle concerne également des procédés de traitement ou de prévention d'infections rétrovirales par administration d'une quantité efficace des inhibiteurs, et des compositions pharmaceutiques comprenant les inhibiteurs. Les procédés comprennent la formation d'un mélange comprenant une séquence linéaire d'un fragment de la tige-boucle de l'anticodon d'ARNt qui n'est pas capable de former une tige-boucle, une molécule d'acide nucléique cible capable de se lier au fragment de tige-boucle de l'anticodon d'ARNt, et un composé à tester. Le mélange est incubé dans des conditions qui permettent la liaison du fragment de tige-boucle de l'anticodon d'ARNt et de la molécule d'acide nucléique cible en l'absence du composé à tester. Des tests peuvent être réalisés pour détecter si le composé à tester inhibe ou non la liaison du fragment de tige-boucle de l'anticodon d'ARNt avec la molécule d'acide nucléique cible.

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