Compositions and methods for the preservation of living tissues

Details Compositions and methods for the preservation of living tissues Compositions and methods for the preservation of living tissues

A - Human Necessities

01

N

A01N 1/00 (2006.01) A01N 1/02 (2006.01) A01N 3/00 (2006.01) A61K 35/12 (2006.01) A61K 35/14 (2006.01) A61K 35/28 (2006.01) C12N 1/04 (2006.01) C12N 5/00 (2006.01)

Patent

CA 2257497

The present invention provides solutions and method for preserving biological material that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of biological activity. The inventive solutions may be either (i) substantially isotonic with the biological material to be preserved and are substantially free of univalent oxyanions and of iodide and/or (ii) comprise a first neutral solute having a molecular weight of at least about 335 and a solubility in water of at least about 0.3 M, and a second neutral solute having a molecular weight of less than about 200 and having both hydrophilic and hydrophobic moieties. The inventive solutions preferably contain CaSO4, together with combinations of anions and cations from the protein-stabilizing ends of the Hofmeister series, such as K2SO4. The invention also encompasses pretreatment of the biological material with sodium butyrate prior to the preservation solution.

La présente invention concerne des solutions et procédés de conservation de matériel biologique, permettant de stocker des organes, tissus et cellules pendant des périodes prolongées avec une perte minimale d'activité biologique de ceux-ci. Les solutions selon invention peuvent être (i) essentiellement isotoniques quant au matériel biologique à conserver et elles sont sensiblement dépourvues d'oxy-anions univalents et d'iodure et/ou (ii) comprennent un premier soluté neutre possédant un poids moléculaire d'au moins 335 et une solubilité dans l'eau d'au moins 0,3 M, ainsi qu'un second soluté neutre possédant un poids moléculaire inférieur à environ 200 et présentant à la fois des fractions hydrophiles et hydrophobes. Les solutions selon invention contiennent, de préférence, CaSO¿4? en même temps que des combinaisons d'anions et de cations provenant d'extrémités de stabilisation de protéines de la série Hofmeister, telles que K¿2?SO¿4?. L'invention concerne également le traitement préalable du matériel biologique à l'aide de butyrate de sodium, avant utilisation de la solution de conservation.

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