C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 7/00 (2006.01) C12N 5/071 (2010.01) C12N 7/01 (2006.01)
Patent
CA 2733399
The present invention relates to virus growth media that improve the yield of alpha-herpesviruses (e.g., HSV-2) grown in cell cultures. The growth media of the invention include two additives, a disaccharide and a lipid mixture, that can be added to serum-free or serum-enriched growth media to improve the efficiency of virus production. The invention further provides methods of producing alpha-herpesviruses (e.g., HSV-2) in such growth media.
La présente invention concerne des milieux de croissance virale qui améliorent le rendement de virus herpès alpha (par exemple, HSV-2) en cultures cellulaires. Les milieux de croissance de linvention comprennent deux additifs, un disaccharide et un mélange de lipides, qui peuvent être ajoutés à des milieux de croissance sans sérum ou enrichis en sérum pour améliorer lefficacité de la production de virus. Linvention concerne en outre des procédés de production de virus herpès alpha (par exemple, HSV-2) dans lesdits milieux de croissance.
Delagrave Simon
Hamberger John
Oubelaid Rachid
Fasken Martineau Dumoulin Llp
Sanofi Pasteur Biologics Co.
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