Compositions and methods for the simultaneous detection and...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07D 219/04 (2006.01) C07H 21/00 (2006.01) C12Q 1/04 (2006.01) C12Q 1/70 (2006.01) G01N 21/76 (2006.01)

Patent

CA 2201595

The invention relates to methods for simultaneously or sequentially detecting multiple nucleic acid analytes in a single medium utilizing oligonucleotide hybridization probes coupled to different chemiluminescent labeling reagents. The methods may be used in a heterogeneous, homogeneous or non-homogeneous assay system. The invention also relates to specific combinations of chemiluminescent labeling reagents suitable, when coupled to an oligonucleotide probe, for use together in methods for the detection of multiple nucleic acid analytes. The invention also concerns kits useful in these methods.

Linvention concerne des procédés de détection simultanée ou séquentielle d'analytes multiples d'acide nucléique dans un seul milieu, à l'aide de sondes d'hybridation oligonucléotidique couplées à différents réactifs de marquage chimioluminescent. On peut utiliser les procédés dans un système d'analyse hétérogène, homogène ou non-homogène. L'invention concerne également des combinaisons spécifiques de réactifs de marquage chimioluminescents adaptés, lorsqu'ils sont couplés à une sonde oligonucléotidique, à une utilisation ensemble dans des procédés de détection d'analytes multiple d'acide nucléique. L'invention concerne également des nécessaires utiles dans ces procédés.

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