Compositions and methods for the use of fmoc derivatives in...

Details Compositions and methods for the use of fmoc derivatives in... Compositions and methods for the use of fmoc derivatives in...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C07H 21/02 (2006.01)

Patent

CA 2500285

Methods of nucleic acid preparation are described, including preparation of mRNA, using FMOC derivatives to synthesize oligonucleotides in addition to applying FMOC protocols to various therapeutic and diagnostic methods. In some embodiments a single stranded oligonucleotide is synthesized bound to a polymer support (such as optic fiber glass filters) using said FMOC derivatives.

L'invention concerne des méthodes de préparation d'acide nucléique, qui consistent à préparer de l'ARNm, à utiliser des dérivées de FMOC pour la synthèse d'oligonucléotides et l'application de protocoles FMOC à diverses méthodes thérapeutiques et diagnostiques. Dans certains modes de réalisation, un oligonucléotide simple brin est synthétisé lié à un support de type polymère (tel que des filtres optiques en fibre de verre) au moyen desdits dérivés de FMOC.

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