Compositions and methods for use in recombinational cloning...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) C07H 21/04 (2006.01) C07K 1/00 (2006.01) C07K 14/00 (2006.01) C12N 1/21 (2006.01) C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12N 15/70 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2363924

The present invention relates generally to compositions and methods for use in recombinational cloning of nucleic acid molecules. In particular, the invention relates to nucleic acid molecules encoding one or more recombination sites or portions thereof, to nucleic acid molecules comprising one or more of these recombination site nucleotide sequences and optionally comprising one or more additional physical or functional nucleotide sequences. The invention also relates to vectors comprising the nucleic acid molecules of the invention, to host cells comprising the vectors or nucleic acid molecules of the invention, to methods of producing polypeptides using the nucleic acid molecules of the invention, and to polypeptides encoded by these nucleic acid molecules or produced by the methods of the invention. The invention also relates to antibodies that bind to one or more polypeptides of the invention or epitopes thereof. The invention also relates to the use of these compositions in methods for recombinational cloning of nucleic acids, in vitro and in vivo, to provide chimeric DNA molecules that have particular characteristics and/or DNA segments.

La présente invention concerne de façon générale des compositions et des méthodes de clonage recombinatoire de molécules d'acide nucléique. Elle concerne en particulier des molécules d'acide nucléique codant tout ou partie d'un ou de plusieurs sites de recombinaison, et concerne également des molécules d'acide nucléique comprenant une ou plusieurs de ces séquences nucléotidiques de sites de recombinaison, et comprenant éventuellement une ou plusieurs séquences nucléotidiques supplémentaires fonctionnelles ou physiques. En outre, cette invention concerne des vecteurs comprenant ces molécules d'acide nucléique, des cellules hôtes comprenant ces vecteurs ou ces molécules d'acide nucléique, des méthodes de production de polypeptides au moyen de ces molécules d'acide nucléique, ainsi que des polypeptides codés par ces molécules d'acide nucléique ou produits à l'aide des méthodes de l'invention. Par ailleurs, cette invention concerne des anticorps se liant à un ou plusieurs polypeptides de l'invention ou à des épitopes de ceux-ci. L'invention concerne également l'utilisation de ces compositions dans des méthodes de clonage recombinatoire d'acides nucléiques, in vitro et in vivo, afin d'obtenir des molécules d'ADN chimères présentant des caractéristiques et/ou segments d'ADN particuliers.

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