Compositions and methods of zearalenone detoxification

Details Compositions and methods of zearalenone detoxification Compositions and methods of zearalenone detoxification

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) A01H 5/00 (2006.01) A01N 63/00 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 9/18 (2006.01) C12N 15/31 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01) C12P 21/02 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2441691

The present invention provides compositions and methods for detoxification of mycotoxins. Compositions include novel nucleotide sequences encoding zearalenone detoxification polypeptides, and biologically active variants thereof. Further provided are methods for mycotoxin detoxification using the sequences disclosed herein. One method comprises stably incorporating into the genome of a plant cell, a nucleotide sequence of the present invention operably linked to a heterologous promoter and regenerating a stably transformed plant that expresses the nucleotide sequence. An additional method comprises incorporating a nucleotide sequence of the present invention operably linked to a heterologous promoter into a micro-organism and applying said micro-organism to a plant or gain.

La présente invention concerne des compositions et des procédés de détoxication de mycotoxines. Lesdites compositions comprennent de nouvelles séquences nucléotidiques codant pour des polypeptides de détoxication de la zéaralenone, et des variants biologiquement actifs de ceux-ci. L'invention concerne également des procédés de détoxication de mycotoxines utilisant les séquences de l'invention. Un procédé comporte l'incorporation de manière stable dans un génome d'une cellule végétale, une séquence nucléotidique de la présente invention liée en fonctionnement à un promoteur hétérologue et la régénération de manière stable de la plante transformée qui exprime la séquence nucléotidique. Un autre procédé comporte l'incorporation d'une séquence nucléotidique de la présente invention liée en fonctionnement à un promoteur hétérologue dans un micro-organisme et l'application dudit micro-organisme à une plante ou graine.

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