Compositions and methods to enhance sensitivity of cancer...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) C07H 21/04 (2006.01) C07K 14/47 (2006.01) C07K 16/00 (2006.01)

Patent

CA 2380507

An isolated nucleic acid sequence of a mitotic checkpoint gene, chfr, encodes a Chfr protein having a Forkhead-associated domain and a Ring Finger. This protein is required for regulation of the transition of cells from prophase to metaphase during mitosis. The chfr nucleic acid and Chfr polypeptide are useful in diagnosing tumorigenic cells and in screening for drugs which can inhibit the activity of Chfr in a cancer cell, thereby rendering the cell more sensitive to additional anti-tumor therapies.

L'invention concerne une séquence d'acide nucléique isolée d'un gène de point de contrôle mitotique, chfr, codant pour une protéine Chfr qui possède un domaine associé à Forkhead et un Ring Finger. Cette protéine est nécessaire à la régulation de la transition de cellules d'une prophase à une métaphase lors de la mitose. L'acide nucléique chfr et le polypeptide de Chfr s'utilisent pour le diagnostic de cellules tumorigènes et le criblage de médicaments capables d'inhiber l'activité de Chfr dans une cellule cancéreuse, ce qui rend la cellule plus sensible à de nouvelles thérapies antitumorales.

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