Compositions and methods to identify haplotypes

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2460652

The present invention relates to the field of genomics and genetic analysis, more particularly to genetic mapping of complex quantitative and qualitative traits. This invention more particularly relates to compositions and methods to identify haplotypes of associated allelic variants in nucleic acid fragments from different sources. The method allows the unambiguous identification of a trait-associated haplotype over large stretches of DNA up to several kilobases. The invention can be used to identify haplotypes related to various conditions or diseases, in particular to the ability of a subject to respond to therapeutic treatments.

La présente invention a trait au domaine de la génomique et de l'analyse génétique, et plus particulièrement à la cartographie génétique de caractères complexes quantitatifs et qualitatifs. Cette invention se rapporte plus particulièrement à des compositions et à des procédés permettant d'identifier des haplotypes de variants allèles associés dans des fragments d'acides nucléiques issus de sources différentes. Le procédé de l'invention permet l'identification non ambiguë d'un haplotype associé à un caractère sur de grandes longueurs d'ADN pouvant contenir jusqu'à plusieurs kilobases. Cette invention peut être mise en oeuvre pour l'identification d'haplotypes associés à divers troubles ou états pathologiques, et en particulier à la capacité d'un sujet à réagir à des traitements thérapeutiques.

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