Compositions and process for amplifying and detecting...

Details Compositions and process for amplifying and detecting... Compositions and process for amplifying and detecting...

C - Chemistry, Metallurgy

12

N

195/1.24, 195/1.

C12N 15/09 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2001110

Abstract of the Disclosure Compositions and methods useful for detecting, amplifying and/or characterizing polynucleotides of interest are disclosed herein. Specifically, this disclosure teaches how to make and use a mobile promoter structure comprising a transcription promoter and a probe sequence. The probe sequence enables the mobile promoter structure to select via hybridization a specific polynucleotide of interest. The promoter enables the transcription of the target polynucleotide. The method of mobile promoter structure- directed transcription can be applied to detect, and/or characterize pathogens, disease states, and genetic traits. While a single mobile promoter structure-directed transcription enables linear amplification, the process can be used in combination with other processes to bring about exponential amplification.

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