Compositions and processes for genotyping single nucleotide...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/16 (2006.01) C12N 9/14 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/42 (2006.01)

Patent

CA 2540875

The invention relates to processes, compositions and kits for analysis of nucleic acid variations, especially single nucleotide polymorphisms (SNPs). An inventive process includes a procedure to enzymatically remove inorganic pyrophosphate from a sample prior to and/or during a single base extension reaction. Processes and compositions described herein are especially useful in nucleic acid analysis methods designed to minimize transfer and separation steps.

L'invention concerne des procédés, compositions et kits permettant d'analyser des variations d'acides nucléiques, plus spécifiquement des polymorphismes d'un nucléotide simple (PNS). Un procédé de cette invention comprend une procédure permettant d'éliminer au niveau enzymatique du pyrophosphate inorganique d'un échantillon avant et/ou durant une réaction d'extension de base simple. Les procédés et compositions de cette invention sont particulièrement utiles dans des procédés d'analyse d'acides nucléiques conçus pour réduire au maximum les étapes de transfert et de séparation.

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