Compositions comprising prfa* mutant listeria and methods of...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/21 (2006.01) A61K 39/02 (2006.01) A61K 39/12 (2006.01)

Patent

CA 2724649

The invention provides recombinant Listeria that constitutively express Prf A and comprise polynucleotides that encode polypeptides such as tumor or infectious agent antigens, operably linked to a PrfA responsive regulatory agent. Methods of using the Listeria, and compositions thereof, to induce or enhance an immune response and/or in the treatment of disease are provided. Methods of producing the bacteria are also provided.

L'invention porte sur des Listeria recombinants qui expriment d'une manière constitutive le PrfA et qui comportent des polynucléotides qui codent pour des polypeptides tels que des antigènes d'agents tumoraux ou infectieux, liés d'une manière opérationnelle à un agent régulateur sensible au PrfA. L'invention concerne des procédés d'utilisation de Listeria, et des compositions de celles-ci pour induire ou améliorer une réponse immune et/ou dans le traitement d'une maladie. L'invention concerne aussi des procédés de production des bactéries.

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