Compositions containing nucleases and chelators to enhance...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/08 (2006.01) C12N 1/02 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2264885

The present invention provides compositions and methods which either prevent cell clumps from forming, or removing clumps which have formed, in cell separating devices. The present compositions contain a nuclease, divalent cations, and chelating agents. Surprisingly, nuclease activity is not decreased in the presence of chelating agents. During cell separation procedures using the present composition, cell yield, purity, and viability are enhanced. Also, kits for use with automated cell separating devices using these compositions are provided.

La présente invention se rapporte à des compositions et à des procédés qui soit permettent d'éviter l'agglutination des cellules soit permettent de supprimer les amas de cellules qui se sont formés dans des dispositifs de séparation de cellules. Les compositions de la présente invention contiennent une nucléase, des cations bivalents et des agents chélateurs. De manière surprenante, l'activité de la nucléase n'est pas réduite en présence des agents chélateurs. Lors des procédures de séparation de cellules au cours desquelles on utilise les compositions de la présente invention, on accroît le rendement, la pureté et la viabilité des cellules. L'invention se rapporte également à des kits destinés à des dispositifs automatisés de séparation de cellules qui font usage de ces compositions.

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