Compositions, devices, systems, and methods for using a...

G - Physics – 01 – N

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G01N 33/483 (2006.01) G01N 27/66 (2006.01)

Patent

CA 2684801

The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore. The devices and methods are also used to determine rapidly (~ >50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

La présente invention concerne des dispositifs et des procédés capables de détecter et de contrôler un polymère individuel dans un mélange, sur lequel agit un autre composé, par exemple, une enzyme, dans un nanopore. Les dispositifs et procédés sont également utilisés pour déterminer rapidement (~ >50 Hz) la séquence de bases nucléotidiques d'un polynucléotide sous rétrocontrôle ou en utilisant les signaux produits par les interactions entre le polynucléotide et le nanopore. L'invention a une utilité particulière dans les domaines de la biologie moléculaire, de la biologie structurale, de la biologie cellulaire, des interrupteurs moléculaires, des circuits moléculaires, et des dispositifs de calcul moléculaire, ainsi que dans leur fabrication.

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