Compositions for bacterial mediated gene silencing and...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/21 (2006.01)

Patent

CA 2591565

Methods are described for the delivery of one or more small interfering RNAs (siRNAs) to a eukaryotic cell using a bacterium. Methods are also described for using this bacterium to regulate gene expression in eukaryotic cells using RNA interference, and methods for treating cancer off cell proliferative disorders. The bacterium includes one or more siRNAs or one or more DNA molecules encoding one or more siRNAs. Vectors are also described for use with the bacteria of the invention for causing RNA interference in eukaryotic cells.

L'invention porte sur: des procédés de transfert d'un ou plusieurs ARNsi (petit ARN interférant) à une cellule eukaryote par l'intermédiaire d'une bactérie; sur des procédés d'utilisation de ladite bactérie pour réguler l'expression génique par interférence d'ARN; sur des procédés de traitement du cancer ou de troubles prolifératifs (la bactérie comportant un ou plusieurs ARNsi ou une ou plusieurs molécules codant pour le ou les ARNsi); et sur des vecteurs utilisés avec la bactérie de l'invention et provoquant une interférence d'ARN dans des cellules eukaryotes.

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