C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2744335
The invention provides highly sensi-tive, specific and efficient quantitative real-time PCR compositions, methods and assay kits to detect at least one IFN subtype and/or IFN subtype allotypic vari-ants. Primer/probe sets complementary to the coding sequence of an IFN subtype of interest avoid spurious detection of degraded mRNA and enhances the corre-lation between the IFN subtype that is measured by the assays of the invention and the protein that is actually expressed. The invention also provides methods for designing primers and methods of using the composi-tions and assay kits. The compositions, kits, and meth-ods of the invention may be used, for example, to monitor vaccine efficacy, autoimmune disease, chronic infections, or tumor therapy.
La présente invention concerne des compositions, procédés et kits dessai de PCR en temps réel quantitative très sensibles, spécifiques et efficaces pour détecter au moins un sous-type dIFN et/ou des variants allotypiques de sous-type dIFN. Des ensembles amorce/sonde complémentaires de la séquence codante dun sous-type dIFN dintérêt évitent la détection erronée dARNm dégradé et augmentent la corrélation entre le sous-type dIFN qui est mesuré par les essais de linvention et la protéine qui est effectivement exprimée. Linvention concerne en outre des procédés pour concevoir des amorces et des procédés dutilisation des compositions et des kits dessai. Les compositions, kits, et procédés de linvention peuvent être utilisés, par exemple, pour contrôler lefficacité dun vaccin, une maladie auto-immune, des infections chroniques, ou une thérapie anticancéreuse.
Mane Viraj Pramod
Rabin Ronald
Borden Ladner Gervais Llp
Departmen T. Of Health And Human Services The United States Of America As Represented By The Secretary
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