Compositions for use in coating of substrate for cell sheet...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/00 (2006.01)

Patent

CA 2539613

A process for producing a cell sheet, comprising culturing cells up to a state of saturation on the surface of a support having its surface coated with fibrin, continuing the culturing for a period of time sufficient to achieve decomposition of the fibrin at cell bottom surface and detaching the cultured cells in the form of a sheet from the support surface. Further, there are provided a support for preparation of cell sheet having its surface coated with fibrin; and a composition for coating the surface of a support for preparation of cell sheet with fibrin, which composition is characterized by containing fibrinogen and thrombin. Cell sheets can be produced by simple operation using a support coated with commercially available familiar material whose safety is ensured.

La présente invention a trait à un procédé de production d'une feuille cellulaire, comprenant la culture de cellules jusqu'à un état de saturation à la surface d'un support dont la surface est revêtue de fibrine, la poursuite de la culture pour une durée suffisante pour obtenir une décomposition de la fibrine à la surface inférieure des cellules et la séparation des cellules cultivées sous la forme d'une feuille de la surface du support. En outre, l'invention a trait à un support pour la préparation de feuille cellulaire présentant une surface revêtue de fibrine ; et à une composition pour le revêtement de la surface d'un support pour la préparation de feuille cellulaire avec de la fibrine, ladite composition étant caractérisée en ce qu'elle contient du fibrinogène et de la thrombine. Des feuilles cellulaires peuvent être produites par une simple opération au moyen d'un support revêtu d'un matériau disponible dans le commerce dont l'innocuité est garantie.

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