Compositions, kit and one-step method for monitoring...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/56 (2006.01)

Patent

CA 2566411

Provided are compositions and methods for accurate determination of the concentration of anticoagulant in a sample such as a blood or plasma sample. The compositions can contain a Factor X compound and Factor V compound, and additional components as well. Methods for performing the assay include one- step methods in which a sample is added to a coagulation assay composition, and time is monitored from the point of adding the sample and coagulation assay composition to an endpoint, such as clot formation.

La présente invention concerne des compositions et des procédés permettant de déterminer de manière précise la concentration d'un anticoagulant dans un échantillon tel qu'un échantillon de sang ou de plasma. Ces compositions peuvent contenir un composé de facteur X et un composé de facteur V, de même que des composants additionnels. Ces procédés permettent d'effectuer le dosage consistant en des procédés en une étape dans laquelle un échantillon est ajouté à une composition de dosage de coagulation et, on surveille le temps à partir de l'instant d'addition de l'échantillon dans la composition de dosage de coagulation à l'instant final, tel que la formation d'un caillot.

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