Compositions, methods, and kits for amplifying nucleic acids

Details Compositions, methods, and kits for amplifying nucleic acids Compositions, methods, and kits for amplifying nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 9/00 (2006.01) C12N 9/12 (2006.01) C12N 9/99 (2006.01) C12P 19/34 (2006.01) C12Q 1/25 (2006.01) C07H 21/00 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2624634

The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided.

Compositions, procédés et kits pour l'amplification d'acides nucléiques cibles avec réduction de la fluorescence non spécifique et des produits d'amplification non désirables, que l'on appelle parfois produits d'amplification secondaires ou produits secondaires parasites. Les inhibiteurs d'enzyme considérés comportent une séquence nucléotidique et au moins un extincteur. On décrit aussi des complexes à inhibiteur d'enzyme associé à une enzyme, sachant qu'au moins une activité enzymatique de l'enzyme est inhibée. On décrit enfin des procédés d'amplification d'acide nucléique cible avec réduction des produits d'amplification non désirables, et des procédés de réduction de la fluorescence non spécifique, et par ailleurs des kits permettant d'optimiser la performance de certains procédés visés.

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