Compositions, methods and kits for immunochemical...

C - Chemistry – Metallurgy – 07 – K

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C07K 16/18 (2006.01) C07K 14/47 (2006.01) G01N 33/564 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2482658

Various antibodies with different binding characteristics for structurally normal or abnormal MBL bound to mannan or to an antibody against MBL are provided. 5 Also provided are methods for selecting antibodies against MBL with different binding characteristics and methods and kits for using these antibodies to measure MBL capable of binding to mannan or to measure normally oligomerized MBL and abnormal, poorly oligomerized MBL in a sample. The methods and kits are useful in diagnosing increased susceptibility to and exacerbation of infections and autoimmune diseases.

L'invention concerne divers anticorps présentant différentes caractéristiques de liaison qui permettant la liaison structurelle de la MBL normale ou anormale à la mannane ou à un anticorps anti-MBL. L'invention concerne en outre des procédés de sélection d'anticorps anti-MBL présentant différentes caractéristiques de liaison ainsi que des procédés et des kits d'utilisation de ces anticorps pour mesurer la MBL capable de se lier à la mannane, ou pour mesurer la MBL normalement oligomérisée ou faiblement oligomérisée dans un échantillon. Ces procédés et kits sont en outre utilisés dans le diagnostic de l'exacerbation et de la susceptibilité accrue aux infections et des maladies auto-immunes.

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