Compositions, methods and kits pertaining to luminescent...

C - Chemistry – Metallurgy – 07 – D

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C07D 487/04 (2006.01) C12Q 1/25 (2006.01) C12Q 1/34 (2006.01) C12Q 1/44 (2006.01) C12Q 1/66 (2006.01) G01N 33/52 (2006.01) C12N 9/02 (2006.01)

Patent

CA 2462506

A method of measuring the enzymatic activity of a luciferase includes contacting a luminogenic protein, such as a luciferase, with a protected luminophore to form a composition; and detecting light produced from the composition. The protected luminophore provides increased stability and improved signal-to-background ratios relative to the corresponding unmodified coelenterazine.

Cette invention concerne un procédé de mesure de l'activité enzymatique d'une luciferase, qui consiste à mettre une protéine lumimogène, telle qu'une luciferase, en contact avec un luminophore protégé pour former une composition, et à détecter la lumière produite par cette composition. Par rapport à la coélenterazine non modifiée, le luminophore protégé présente une stabilité accrue et des rapports signal-fond améliorés..

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