Compositions, methods and systems for the discovery of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) C07K 14/195 (2006.01) C07K 14/435 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C07K 16/40 (2006.01) C12N 9/00 (2006.01) C12N 9/10 (2006.01) C12N 9/16 (2006.01) C12N 15/10 (2006.01) C12N 15/12 (2006.01) C12N 15/31 (2006.01) C12N 15/52 (2006.01) C12N 15/55 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01) C12Q 1/68 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2445692

Five protein families cooperate to form the warhead structure that characterizes enediyne compounds, both chromoprotein enediynes and non-chromoprotein enediynes. The protein families include a polyketide synthase and thioesterase protein which form a polyketide synthase catalytic complex involved in warhead formation in enediynes. Genes encoding a member of each of the five protein families are found in all enediyne biosynthetic loci. The genes and proteins may be used in genetic engineering applications to design new enediyne compounds and in methods to identify new enediyne biosynthetic loci.

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