Compositions of engineered human arginases and methods for...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/50 (2006.01) A61K 38/48 (2006.01) A61P 35/00 (2006.01) C07K 19/00 (2006.01) C12N 15/57 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2742497

Compositions and methods for the treatment of cancer are described, and, more preferably, to the treatment of cancers that do not express, or are otherwise deficient in, argininosuccinate synthetase, with enzymes that deplete L-Arginine in serum. In one embodiment, the present invention contemplates an arginase protein, such as a human Arginase I protein, comprising at least one amino acid substitution and a metal cofactor, said protein comprising an increased catalytic activity when compared with a native human Arginase I.

Les compositions et les méthodes ci-décrites sont destinées à traiter le cancer, et plus préférablement, à traiter les cancers qui n'expriment pas, ou qui, pour toute autre raison, ont un déficit d'argininosuccinate synthétases, avec des enzymes qui épuisent la L-arginine sérique. Dans un mode de réalisation, cette invention concerne une protéine arginase, telle qu'une protéine arginase I humaine, comprenant au moins une substitution d'acide aminé et un cofacteur métallique, ladite protéine ayant une activité catalytique accrue, comparée à une arginase I humaine native.

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