Compositions of melanoma differentiation associated gene-7...

Details Compositions of melanoma differentiation associated gene-7... Compositions of melanoma differentiation associated gene-7...

C - Chemistry, Metallurgy

12

N

C12N 15/19 (2006.01) A61K 39/395 (2006.01) A61K 48/00 (2006.01) C07K 14/54 (2006.01) C07K 16/24 (2006.01) C07K 16/32 (2006.01) C12N 5/10 (2006.01) C12N 15/12 (2006.01) C12N 15/63 (2006.01) C12N 15/85 (2006.01) C12Q 1/68 (2006.01) G01N 33/574 (2006.01) C40B 50/06 (2006.01)

Patent

CA 2174354

The invention relates to an isolated mda-7 nucleic acid encoding the MDA-7 amino acid sequence of SEQ ID NO:7, the use of the mda-7 gene in the preparation of a pharmaceutical composition and a method of detecting the expression of said gene.

Procédé de constitution d'une banque d'ADNc à soustraction d'une cellule, consistant à: (a) créer la banque d'ADNc de la cellule; (b) isoler les ADN double brin de la banque d'ADNc; (c) libérer les inserts d'ADNc double brin des ADN double brin; (d) dénaturer les inserts d'ADN double brin isolés; hybrider les inserts d'ADNc double brin dénaturés à l'aide de molécules marquées d'un acide nucléique monobrin à soustraire de la banque d'ADNc; et (f) séparer les molécules marquées d'acide nucléique monobrin hybridé des inserts d'ADNc double brin de façon à constituer la banque d'ADNc à soustraction d'une cellule. L'invention porte également sur différentes utilisations de ladite banque.

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