Compositions of prokaryotic phenylalanine ammonia-lyase and...

A - Human Necessities – 61 – K

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A61K 38/16 (2006.01) A61P 35/00 (2006.01)

Patent

CA 2687028

The present invention is directed to phenylalanine ammonia-lyase (PAL) variants produced by prokaryotes, wherein such prokaryotic PAL variant has a greater phenylalanine-converting activity and/or a reduced immunogenicity as compared to a wild- type PAL. The invention provides compositions of prokaryotic PAL and biologically active fragments, mutants, variants or analogs thereof, as well as methods for the production, purification, and use of such compositions for therapeutic purposes, including the treatment of cancer.

L'invention concerne des variants de phénylalanine ammoniac/lyase (PAL) produits par des procaryotes, de tels variants procaryotiques de PAL ayant une plus grande activité de conversion et/ou une immunogénicité réduite par rapport à un de type sauvage. L'invention fournit des compositions de PAL procaryotique de fragments, mutants, variantes ou analogues biologiquement actifs et celui-ci, ainsi que des procédés pour la production, la purification, et l'utilisation de telles compositions à des fins thérapeutiques, y compris le traitement du cancer.

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