C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12P 19/34 (2006.01)
Patent
CA 2203143
Compound microsatellites used as self-anchoring primers for screening genomes to detect polymorphisms are provided. The preferred compound microsatellite primers are perfect compound dinucleotide primers wherein the adjacent dinucleotide pairs contain a common nucleotide which is in-phase across the compound junction. In a useful embodiment, these in-phase compound primers have been adapted to other known screening assays including variations of the AFLP assays.
L'invention concerne des microsatellites composés qui sont utilisés en tant que sondes d'autofixation pour la recherche de génomes dans le but de détecter des polymorphismes. Les sondes de microsatellites composées préférées sont des sondes de dinucléotides composés parfaits dans lesquelles les paires de dinucléotides adjacents contiennent un nucléotide commun qui est en phase sur la jonction du composé. Dans un mode de réalisation utile, ces sondes composées en phase ont été adaptées à d'autres dosages d'investigation connus y compris les variations des dosages AFLP.
Morgante Michele
Vogel Julie Marie
Bennett Jones Llp
E.i. Du Pont de Nemours And Company
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