C - Chemistry – Metallurgy – 07 – C
Patent
C - Chemistry, Metallurgy
07
C
C07C 245/10 (2006.01) C07H 19/06 (2006.01) C07H 19/16 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2601554
The invention provides a novel method of labeling oligonucleotides, with reporter moieties, including but not limited to, quenchers, fluorophores, biotin, digoxigenin, peptides and proteins. In addition, this invention provides a method of detecting hybridization of oligonucleotides. This invention also provides novel azo quenchers having the general formula shown below. -Ry R4" R6 The invention further provides compositions comprising labeled oligonucleotides and solid supports. The invention also provides kits comprising at least one composition of the present invention.
L'invention se rapporte à un nouveau procédé de marquage d'oligonucléotides au moyen de fragments rapporteurs comprenant notamment des extincteurs, des fluorophores, de la biotine, de la digoxigénine, des peptides et des protéines. L'invention se rapporte également à un procédé d'hybridation des oligonucléotides. L'invention concerne également de nouveaux extincteurs azoïques représentés par la formule générale décrite ci-après. L'invention concerne également des compositions contenant des oligonucléotides marqués et des supports solides. L'invention se rapporte également à des kits comprenant au moins une composition de ladite invention.
Behlke Mark
Laikhter Andrei
Podyminogin Mikhail
Walder Joseph A.
Yong Yawfui
Integrated Dna Technologies Inc.
Torys Llp
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