C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/90 (2006.01) A01K 67/027 (2006.01) A61K 31/70 (2006.01) A61K 48/00 (2006.01) C07H 21/00 (2006.01) C07K 14/82 (2006.01) C12N 15/79 (2006.01) C12N 15/80 (2006.01)
Patent
CA 2178729
The present invention concerns a polynucleotide having both ribonucleotides and deoxyribonucleotides in a first strand and solely deoxyribonucleotides in a second strand wherein the strands are Watson-Crick paired and are linked by an oligonucleotide so that the polynucleotide has at most a single 3' and a single 5' end. These ends can be ligated so that the polynucleotide is a single continuous circular polymer. The polynucleotide can be used to induce specific alterations in targeted genes.
L'invention concerne un polynucléotide possédant à la fois des ribonucléotides et des désoxyribonucléotides dans un premier brin et uniquement des désoxyribonucléotides dans un deuxième brin; les brins sont assemblés en paires de Watson-Crick et sont reliés par un oligonucléotide, de telle manière que le polynucléotide possède au plus une extrémité unique 3' et une extrémité unique 5'. Ces extrémités peuvent être ligaturées, de façon à ce que le polynucléotide soit un polymère circulaire et continu unique. On peut utiliser ce polynucléotide, afin de provoquer des modifications spécifiques dans des gênes ciblés.
Osler Hoskin & Harcourt Llp
Thomas Jefferson University
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