Compounds and reagent compositions and their use in the...

C - Chemistry – Metallurgy – 09 – B

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150/13, 260/282,

C09B 15/00 (2006.01) G01N 21/64 (2006.01) G01N 33/52 (2006.01) G01N 33/80 (2006.01)

Patent

CA 2024166

Quaternized derivatives of acridine orange and reagents incorporating such derivatives and their use in quantitatively determining reticulocyte levels in whole blood specimen by fluorescence flow cytometry techniques are disclosed. The quarternized derivatives of acridine orange are of the general formula: Image wherein Y is Br? or I? wherein X may be R1 and/or R2 substituted benzyl group Image R1 is hydrogen or fluorine, and R2 is fluorine, trifluoromethyl or hydrogen, or X may be hydroxyl ethylene.

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