Computer-directed assembly of a polynucleotide encoding a...

C - Chemistry – Metallurgy – 07 – H

Patent

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C07H 21/00 (2006.01) C07K 1/00 (2006.01) C12N 15/10 (2006.01) C12N 15/63 (2006.01) C12N 15/66 (2006.01) C12P 19/34 (2006.01) G06F 15/00 (2006.01) G06F 17/00 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2433463

The present invention outlines a novel approach to utilizing the results of genomic sequence information by computer-directed polynucleotide assembly based upon information available in databases such as the human genome database. Specifically, the present invention may be used to select, synthesize and assemble a novel, synthetic target polynucleotide sequence encoding a target polypeptide. The target polynucleotide may encode a target polypeptide that exhibits enhanced or altered biological activity as compared to a model polypeptide encoded by a natural (wild-type) or model polynucleotide sequence.

L'invention décrit une nouvelle approche d'utilisation des informations relatives à la séquence génomique résultant d'un assemblage de polynucléotide commandé par ordinateur fondé sur des informations disponibles dans des bases de données telles que la base de donnée du génome humain. Plus particulièrement, cette invention peut être utilisée afin de sélectionner, de synthétiser et d'assembler une nouvelle séquence polynucléotide cible synthétique codant un polypeptide cible. Le polynucléotide cible peut coder un polypeptide cible qui inhibe une activité biologique améliorée ou altérée par rapport à un polypeptide de référence codé par une séquence polynucléotide naturelle (de type sauvage) ou de référence.

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