Concentration and enrichment of microbial cells and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 1/00 (2006.01) C11D 1/66 (2006.01) C12N 1/06 (2006.01) C12N 1/14 (2006.01) C12N 1/16 (2006.01) C12N 1/20 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2693565

The present invention relates to a method for isolating microorganisms and/or microorganisms nucleic acids from a bodily fluid that may comprise or may be suspected to comprise microorganisms and/or host cells and/or host cells debris. Microor-ganisms nucleic acids may further be isolated by lysing the isolated microorganisms. The present invention also relates to a method for detecting microorganisms in a bodily fluid. The present invention further relates to a saponin formulation and its use.

La présente invention porte sur un procédé d'isolement des microorganismes et/ou des acides nucléiques de microorganismes à partir d'un fluide corporel qui peut comprendre ou peut être soupçonné comprendre des microorganismes et/ou des cellules hôtes et/ou des débris de cellules hôtes. Des acides nucléiques de microorganismes peuvent en outre être isolés par lyse des microorganismes isolés. La présente invention porte également sur un procédé de détection de microorganismes dans un fluide corporel. La présente invention porte en outre sur une formulation de saponine et son utilisation.

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