Conditional knockout method for gene trapping and gene...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12N 15/85 (2006.01) C12Q 1/00 (2006.01)

Patent

CA 2428972

A method for conditionally knocking out and altering gene function and genetic sequences that can be used in such methods, for use in gene trapping and gene targeting. Specifically, the genetic sequence is a inducible gene silencer comprising: (a) a splice acceptor sequence; (b) an internal ribosomal entry site (IRES) sequence; (e) a nucleotide sequence coding for a reporter protein; (d) a polyadenylation sequence; and (e) a pair of oppositely oriented recombination site sequences, which cause single cycle inversions in the presence of a suitable recombinase enzyme, flanking elements (a) through (d).

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