Conditioned cell culture media and uses thereof

Details Conditioned cell culture media and uses thereof Conditioned cell culture media and uses thereof

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01) A61K 8/64 (2006.01) A61K 38/00 (2006.01) A61K 38/18 (2006.01) A61K 38/39 (2006.01) A61K 38/43 (2006.01) A61K 38/44 (2006.01) A61Q 19/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C07K 1/00 (2006.01) C07K 14/52 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2452865

The invention relates to compositions comprising cell culture medium conditioned by cells grown in three-dimensional culture. The cells used to condition the medium may be genetically modified to alter the concentration of growth factors and antioxidants in the medium. The conditioned cell medium (conditioned medium) may be used for at least one of cosmetic applications, cosmeceutical applications, and pharmaceutical applications, among other things. The invention also relates to proteins comprising a heterologous sequence that enhances cell penetration. The invention also relates to cells comprising DNA encoding such proteins. Methods for preparing the inventive compounds are also provided.

L'invention concerne des compositions comprenant un milieu de culture cellulaire conditionné par des cellules obtenues par culture tridimensionnelle. Les cellules utilisées pour le conditionnement du milieu peuvent avoir subi des modifications génétiques visant à modifier la concentration en facteur de croissance et en antioxydants du milieu. Ce milieu cellulaire conditionné (milieu conditionné) peut être utilisé dans au moins une des applications suivantes : applications cosmétiques, applications cosméceutiques, et applications pharmaceutiques et autres. L'invention concerne également des protéines comprenant une séquence hétérologue qui améliore la pénétration cellulaire, ainsi que des cellules contenant l'ADN codant pour de telles protéine. L'invention concerne en outre des procédés permettant de préparer les composés décrits.

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