Conditioned media and methods of making a conditioned media

Details Conditioned media and methods of making a conditioned media Conditioned media and methods of making a conditioned media

C - Chemistry, Metallurgy

12

N

C12N 1/00 (2006.01)

Patent

CA 2747727

A stable and scalable process is provided to manufacture reduced serum umbilical cord tissue-derived (UTC)-con-ditioned media. The method includes the culture of a UTC under reduced serum conditions. Subsequently, the UTC is washed and grown in serum-free basal media. After approximately 24 hours, the conditioned media is collected, filtered and concentrated by use of an approximately 5 kDa or similar cut-off membrane.

La présente invention concerne un procédé stable et évolutif pour fabriquer des milieux conditionnés dérivés de tissu de cordon ombilical en conditions de sérum réduites. Le procédé comprend la culture d'un tissu de cordon ombilical dans des conditions de sérum réduites. Ensuite, le tissu de cordon ombilical est lavé et cultivé dans un milieu de base sans sérum. Après approximativement 24 heures, le milieu conditionné est recueilli, filtré et concentré par l'utilisation d'une membrane à seuil de coupure d'environ 5 kDa ou similaire.

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