Conditioning biomass for microbial growth

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 1/22 (2006.01) C12N 9/02 (2006.01) C12N 9/42 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2674295

The present invention relates to methods for improving the yield of microbial processes that use lignocellulose biomass as a nutrient source. The methods comprise conditioning a composition comprising lignocellulose biomass with an enzyme composition that comprises a phenol oxidizing enzyme. The conditioned composition can support a higher rate of growth of microorganisms in a process. In one embodiment, a laccase composition is used to condition lignocellulose biomass derived from non-woody plants, such as corn and sugar cane. The invention also encompasses methods for culturing microorganisms that are sensitive to inhibitory compounds in lignocellulose biomass. The invention further provides methods of making a product by culturing the production microorganisms in conditioned lignocellulose biomass.

La présente invention concerne des procédés permettant d'améliorer le rendement de processus microbiens qui utilisent la biomasse de lignocellulose comme source de nutriments. Ces procédés consistent à conditionner une composition contenant de la biomasse de lignocellulose avec une composition enzymatique qui renferme une enzyme d'oxydation du phénol. La composition conditionnée peut supporter un taux supérieur de croissance de micro-organismes au cours d'un processus. Dans un mode de réalisation, une composition à base de laccase est utilisée pour conditionner la biomasse de lignocellulose dérivée de plantes sans bois, telles que le maïs et la canne à sucre. Cette invention a aussi pour objet des procédés permettant de mettre en culture des micro-organismes sensibles aux composés inhibiteurs de la biomasse de lignocellulose, ainsi que des procédés permettant d'élaborer un produit par culture des micro-organismes de production dans la biomasse de lignocellulose conditionnée.

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