Confocal microscope

Details Confocal microscope Confocal microscope

G - Physics

02

B

88/21, 88/94

G02B 21/00 (2006.01) G02B 21/02 (2006.01) G02B 21/06 (2006.01) G02B 21/18 (2006.01) G02B 26/10 (2006.01)

Patent

CA 1325537

ABSTRACT A diffraction limited confocal microscope is disclosed. The microscope includes an energy source to provide focussable illuminating energy and a single mode energy guide comprising a core, an energy receiver and an energy exit. The energy guide is operatively associated with the energy source so that illuminating energy from the energy source is received by the energy receiver and coupled into the core and guided to the energy exit so as to emerge from the core at the energy exit. A first focusser is operatively associated with the energy exit to focus at least a portion of the illuminating energy emerging from the core into a diffraction limited spot pattern volume having a central portion which in use intersects an object. A second focusser operatively associated with the first focusser, and in use with the object, to collect outgoing energy from the volume resulting from interaction between the illuminating energy in the volume and the object and/or resulting from transmission of illuminating energy through the volume. The microscope includes a detector having an aperture and a detecting element wherein the detector is operatively associated with the second focusser whereby the second focusser images the aperture onto the central portion wherein the numerical aperture, NA, of the outgoing energy originating from the central portion focussed onto the aperture, the wavelength of the outgoing energy, .lambda., and the average diameter, d, of the aperture are related by the equation: NA ? 0.6 x .lambda. / a whereby the detector detects the outgoing energy.

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