Confocal microscope

G - Physics – 02 – B

Patent

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G02B 21/00 (2006.01)

Patent

CA 2246698

A confocal microscope comprises a light source, focusing means, a detector and an encoded mask (17). The light source illuminates the mask (17) and the encoded light is then focused on a specimen. Light the from the specimen is then decoded, by either the same or a separate complementary mask, before being detected, for example by a camera. The mask (17) is encoded with a pattern which generates a combined confocal and non-confocal image and the confocal image is subsequently extracted from the combined image. This means that confocal images of different regions of the specimen can be produced simultaneously which in turn enables real-time confocal imaging of the specimen. In addition, as the microscope is no longer restricted to delta correlated images, an improvement in the light budget can also be achieved.

Un microscope confocal comprend une source de lumière, un moyen de focalisation, un détecteur et un masque codé (17). La source de lumière éclaire le masque (17) et la lumière codée est ensuite focalisée sur un échantillon. La lumière de l'échantillon est ensuite décodée, par le même masque ou par un masque complémentaire séparé, avant d'être détectée, par exemple, par une caméra. Le masque (17) est codé avec un motif qui génère une image combinée confocale/non confocale et l'image confocale est extraite subséquemment de l'image combinée. Ceci signifie que les images confocales des différentes régions de l'échantillon peuvent être produites simultanément, ce qui permet d'obtenir une image confocale de l'échantillon en temps réel. En outre, comme le microscope n'est plus limité à des images à corrélation delta, la lumière se trouve mieux utilisée.

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